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The antibody is from purified rabbit serum by affinity purification through sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
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Overexpression of this gene is observed in several tumors, which leads to chromosome instability, and therefore might add to tumorigenesis. This protein was observed to associate with, and be linked to, the phosphorylation of NPAT protein (nuclear protein mapped into the ATM locus), which participates in cell-cycle controlled histone gene expression and performs a important part in advertising and marketing cell-cycle development while in the absence of pRB. Two alternatively spliced transcript variants of this gene, which encode distinctive isoforms, are actually described. Two further splice variants were being claimed but detailed nucleotide sequence info is just not still obtainable.
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Blocking peptides are peptides that bind especially towards the focus on antibody and block antibody binding. These peptide usually incorporates the epitope regarded by the antibody.
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This manual outlines further feasible optimization of mobile and immunoassay parameters to be sure the absolute best outcomes are...
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This guideline outlines further probable optimization of mobile and immunoassay parameters to ensure the best possible outcomes are...
Antibodies certain to the blocking peptide now not bind to the epitope to the focus on protein. By evaluating the staining through the blocked antibody compared to the antibody on your own, one can see which staining is specific.
Concentrate on Facts Cyclin E belongs for the extremely conserved cyclin family members, whose members are characterised by a remarkable periodicity in protein abundance through the mobile cycle. Cyclins perform as regulators of CDK kinases. Distinct cyclins show distinct expression and degradation patterns which contribute into the temporal coordination of every mitotic function. Cyclin E forms a posh with and features as being a regulatory subunit of CDK2, whose exercise is website needed for cell cycle G1/S changeover. This protein accumulates within the G1-S period boundary and it is degraded as cells development by means of S phase.
The measurement of protein phosphorylation is a useful gizmo for measuring the modulation of receptor activation by equally antibodies...
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